30/03/1311تاريخ پذيرش 30/11/1311تاريخ دريافت

a-TNF1B-ILDRG

STZ DRG

TNF-aIL-1BDRGp=0.001

TNF-a

p=0.001

TNF-aDRG

TNF-aIL-1B

Email: khosrojalali@gmail.com

Dorsal Root Ganglia

Streptozotocin

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C

DRG

TNF-αIL-

1B

DRG

Zhu

a-TNFDRG

IL-1BIL-1B

ROS

(4-allyl-2-metoxoxhenhe

Spinal cord

ROS

TNF-aIL1B

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DRGTNF-aIL-1B

kgmg /

DMSO

ml

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H&E

ZEISS

Image J(Image

J Software, NIH)

Real Time PCR

RNA

RNA

DEPC

RNA

Vortex

RNA

cDNA

Fermentas, USAcDNA

TNF-aIL-1B Real

time-PCR

(GAPDH)

-ΔΔCT2

TNF-aIL-1BGAPDH

NCBI

5ˈ-3ˈ

NM_012675.3 F5' GAGATGTGGAAATGGCAGAGGA 3'

R5' GAGAAGATGATGTGAGTGTGAGG 3'

TNF-a

NM_031512.2 F5' TGTGACTGGTGGGATGATGA 3'

R5' GTTCTGTCTATTGAGGTGGAGA3'

IL-1B

XM_017593963.1 F 5' CAT ACT CAG CAC CAG CAT CAC C 3'

R5' AAG TTC AAC GGC ACA GTC AAG G 3'

GAPDH

ANOVA

SPSS

≤P

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DRG

H&E

DRGp=0.001

p=0.001

p=0.01p=0.001

. اندشدهف استاندارد نشان داده انحرا ±ن يانگيم صورتبه هاداده ب(.) يونيگانگل يهاسلولو تعداد ()الف DRGک يستولوژيرات هييتغ (:1)شکل

نشان دهنده ** و با گروه کنترل سهيمقادر (p≤0.05) دار ي* نشان دهنده تفاوت معنموش در هر گروه. 21 يمده از بررسآ دستبهنتابج

است. يابتيمدل دسه با گروه يدر مقا (p≤0.05) دار يتفاوت معن

DRG

mRNA TNF-a

p=0.001

TNF-ap=0.001

05

101520253035404550

*

*

*

**

**

الف

ب

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mRNA IL-1B

DRG

p=0.001

mRNA IL-1BDRG

TNF-aIL-1B

p≤0.05

p≤0.05

0

0.0002

0.0004

0.0006

0.0008

0.001

0.0012

0.0014

0.0016

0.0018

دهش

ل ما

ر نن

ژن

یاب

****

0

0.001

0.002

0.003

0.004

0.005

0.006

0.007

0.008

دهش

ل ما

ر نن

ژن

یاب

**

*

*

*

*

*

ب

الف

**

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TNF-a

IL-1BSTZ

IL1-α)IL1-β

IL-2Il-4IL-6

IL-10IFN-γTGF-

β)TNF-αDRG

TNF-α

TNF-

aIL-1B

DRG

TNF-αIL1-β

DRG

TNF-a

IL-B

IL-6

IL-10 IL-

1RAIL-6

IL-6

TNF-α

IL-6DRG

PIL-6TNF-

αNR1IL-1βP

TNF-αIL1-β

DRG

DRG

TNF-

aDRG

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IL-1B

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Studies in Medical Sciences, Vol. 31(5), August 2020

Original Article

THE EFFECT OF 8 WEEKS OF AEROBIC EXERCISE WITH NANO

EUGENOL SUPPLEMENTATION ON INFLAMMATORY FACTORS

OF TNF-A AND IL-1B AND HISTOLOGICAL CHANGES OF DORSAL

ROOT GANGLION IN DIABETIC RATS

Abbas Shareghi boroujeni 1, khosro Jalali Dehkordi *2, Gholamreza sharifi 3,

Farzaneh Taghian 4, Zohreh Mazaheri5

Received: 19 February, 2020; Accepted: 19 June, 2020

Abstract Background & Aims: The aim of this study was to investigate the effect of 8 weeks of aerobic exercise

with Nano eugenol supplementation on inflammatory factors of TNF-a and IL-1B and histological

changes of dorsal root ganglion in diabetic rats.

Materials & Methods: 25 Wistar 8-week-old male rats were divided into 5 groups: Normal control

group, Diabetic control group (Model), Diabetic + exercise group (Model + Exe), Diabetic group + Nano

vaginal (Model + Nano), and Diabetic + exercise training + nano eugenol (Model + Exe + Nano). The

diabetes model was induced by peritoneal injection of streptozotocin to the respective groups. The

eugenol supplement was also gavaged to the supplement groups. Exercise groups also exercised for 8

weeks, 5 days a week (30 m / min).

Results: Induction of diabetes using STZ led to destruction of the tissue and cell alignment in the DRG

region. Gene changes also showed that TNF-a and IL-1B inflammatory factors showed a significant

increase in the DRG region in the diabetic group compared to the control group (p = 0.001 for both

variables). The study of therapeutic modalities also showed that only the diabetic + exercise +

nanougenol group showed a significant decrease in TNF-a compared to the diabetic group (p = 0.001).

Conclusion: According to the results of the present study, it seems that the use of nanougenol

supplementation along with exercise training may be effective in controlling the neurological damage

of diabetes by negatively regulating the inflammatory factor TNF-a, especially in the DRG region.

Keywords: Aerobic Exercise, Nano eugenol, TNF-a, IL-1B, Diabetes

Address: Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

Tel: +989131854997

Email: khosrojalali@gmail.com

SOURCE: STUD MED SCI 2020: 31(5): 409 ISSN: 2717-008X

PhD student, Department of Sport Physiology, School of Sport Sciences, Isfahan (Khorasgan) Branch, Islamic

Azad University, Isfahan, Iran

Assistant Professor, Department of Sport Physiology, School of Sport Sciences, Isfahan (Khorasgan) Branch,

Islamic Azad University, Isfahan, Iran (Corresponding Author)

Associate Professor, Department of Sport Physiology, School of Sport Sciences, Isfahan (Khorasgan) Branch,

Islamic Azad University, Isfahan, Iran

Associate Professor, Department of Sport Physiology, School of Sport Sciences, Isfahan (Khorasgan) Branch,

Islamic Azad University, Isfahan, Iran

Histogenic Laboratory, Tehran, Iran

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